Fig. 3. TRPM2 downregulation inhibits the in vitro inavsion ability of NSCLC cells. (A & B) Gap closure (wound healing assay) migration analysis of A549 cells before and after TRPM2 silencing. Bar graph shows the statistical analysis of resulted data. (C & D) The quantitative analysis of migration and invasion ability of A549 scramble and TRPM2 KD cells. (E & F) RT-qPCR and western blot analysis of the expression level of EMT markers (t test vs scr. ***, p<0.001; **, p<0.01; * p<0.05).